Gallbladder cancer (GBC) is an aggressive and lethal malignancy with a poor prognosis. Long noncoding RNAs (lncRNAs) and natural products have emerged as key orchestrators of cancer pathogenesis through widespread dysregulation across GBC transcriptomes. Functional studies have revealed that lncRNAs interact with oncoproteins and tumor suppressors to control proliferation, invasion, metastasis, angiogenesis, stemness, and drug resistance. Curcumin, baicalein, oleanolic acid, shikonin, oxymatrine, arctigenin, liensinine, fangchinoline, and dioscin are a few examples of natural compounds that

Capsaicin is the primary capsaicinoid compound responsible for the spiciness of chilli peppers. Several known and unknown genes synthesize capsaicin through various metabolic pathways, such as the phenylpropanoid or the L-valine metabolism pathways. We conducted comprehensive comparative genomics and proteomics analyses to identify genes and proteins associated with the capsaicin pathway in Capsicum chinense, Capsicum baccatum and the two C.annuum cultivars, CM334 and ECW. A BLAST search against the NCBI database identified 26 and 58 enzyme genes and proteins, respectively. These enzyme genes

Chickpea (Cicer arietinum) is a grain crop that is an important source of protein, vitamins, carbohydrates and minerals. It is highly sensitive to salt stress, and salt damage to cellular homeostasis and protein folding affects production. Plants have several mechanisms to prevent cellular damages under abiotic stresses, such as proteins in the endoplasmic reticulum (protein isulfide somerases (PDIs) and PDI-like proteins), which help prevent the build-up of mis-folded proteins that are damaged under abiotic stresses. In this study, we completed initial comprehensive genome-wide analysis of

Domesticated strawberry is susceptible to sudden frost episodes, limiting the productivity of this cash crop in regions where they are grown during early spring. In contrast, the ancestral woodland strawberry (Fragaria vesca) has successfully colonized many habitats of the Northern Hemisphere. Thus, this species seems to harbour genetic factors promoting cold tolerance. Screening a germplasm established in the frame of the German Gene Bank for Crop Wild Relatives, we identified, among 70 wild accessions, a pair with contrasting cold tolerance. By following the physiological, biochemical

Aim The aim of this research was to study serum vitamin D and micro-ribonucleic acid-146a (miRNA-146a) expression in dry eye disease (DED) associated with rheumatoid arthritis (RA) and their clinical correlations to DED parameters. Setting and design This is an observational cross-sectional study that was conducted at Cairo University, Cairo, Egypt. Patients and methods The study participants were divided into three groups: group A, DED/RA (n=35); group B, non-DED/RA (n=36); and group C, non-DED/non-RA (n=35). All participants were assessed for ocular surface disease index, tear breakup time

The dog was probably the first domesticated animal. Despite extensive archaeological and genetic investigations, the origin and the evolution of the extant dogs are still being debated. Dog breeds that have over time been selected for hunting share common ancestral traits. This study represents the first comprehensive attempt to survey at the genomic and mitochondrial level eight hound-like dogs breeds indigenous to the Mediterranean Basin to determine if they share common ancient origins. Results from the microsatellite analysis indicate that all the dog populations have a low inbreeding

Diabetes is a common chronic metabolic disease with a wide range of clinical symptoms and consequences and one of the main causes of death. For the management of diabetes, painless and continuous interstitial fluid (ISF) glucose monitoring is ideal. Here, we demonstrate continuous diabetes monitoring using an integrated microneedle (MN) biosensor with an emergency alert system. MNs are a novel technique in the field of biomedical engineering because of their ability to analyze bioinformation with minimal invasion. In this work we developed a poly(methyl methacrylate) (PMMA) based MN glucose

Nano zero-valent iron (nZVI), bimetallic Nano zero-valent iron-copper (Fe 0- Cu), and fava bean activated carbon-supported with bimetallic Nano zero-valent iron-copper (AC-F e 0-Cu) were prepared and characterized by DLS, FT-IR, XRD, and SEM. The influence of the synthesized adsorbents on the adsorption and removal of soluble anionic methyl orange (M.O) dye was investigated using UV-V spectroscopy. The influence of numerous operational parameters was studied at varied pH (3–9), time intervals (15–180 min), and dye concentrations (25–1000 ppm) to establish the best removal conditions. The

【Objective】 The exploration of germplasm and gene resources in rice for high nitrogen efficiency, along with the elucidation of their molecular mechanisms and genetic effects, represents a significant focus and goal within current research efforts on rice nitrogen use efficiency (NUE).【Method】 To identify the variant loci and candidate genes associated with rice NUE, we collected 190 Asian rice accessions as an association population. After thorough filtering and screening, we obtained 3, 934, 195 high-quality single nucleotide polymorphisms (SNPs). Under field conditions, two nitrogen

Introduction: Soil polluted with Nickel (Ni) adversely affects sunflower growth resulting in reduced yield. Counterbalancing Ni toxicity requires complex molecular, biochemical, and physiological mechanisms at the cellular, tissue, and whole plant levels, which might improve crop productivity. One of the primary adaptations to tolerate Ni toxicity is the enhanced production of antioxidant enzymes and the elevated expression of Ni responsive genes. Methods: In this study, biochemical parameters, production of ROS, antioxidants regulation, and expression of NRAMP metal transporter genes were