Direct detection of biotinylated proteins (DiDBiT) is a proteomic method that can enrich and detect newly synthesized proteins (NSPs) labeled with bio-orthogonal amino acids with 20-fold improved detectability compared to conventional methods. However, DiDBiT has currently been used to compare only two conditions per experiment. Here, we present DiDBiT-TMT, a method that can be used to quantify NSPs across many conditions and replicates in the same experiment by combining isobaric tandem mass tagging (TMT) with DiDBiT. We applied DiDBiT-TMT to brain slices to determine changes in the de novo

The MYB transcription factor (TF) are among the largest gene families of plants being responsible for several biological processes. The R2R3-MYB gene family are integral player regulating plant primary and secondary metabolism, growth and development, and responses to hormones and stresses. The phylogenetic analysis combined with gene structure analysis and motif determination resulted in division of R2R3-MYB gene family into 27 subgroups. Evidence generated from synteny analyses indicated that CqR2R3-MYBs gene family is featured by tandem and segmental duplication events. On the basis of RNA

As an important macro element for all living cells, phosphorus is essential in agricultural production systems and is required in large quantities by elite varieties of crops to maintain yields. Approximately 70% of the worldwide cultivated land suffers from phosphorous deficiency, and it has recently been estimated that the worldwide phosphorous resources will be shattered by the end of this century, thereby increasing the need to develop phosphorus-efficient crops. A greater understanding of how plants can maintain yield with lower phosphorous availability is highly desirable to both

Gastric cancer (GC) remains a major public health challenge worldwide. Long non-coding RNAs (lncRNAs) play important roles in the development, progression, and resistance to the treatment of GC, as shown by recent developments in molecular characterization. Still, an in-depth investigation of the lncRNA landscape in GC is absent. However, The objective of this systematic review is to evaluate our present understanding of the role that lncRNA dysregulation plays in the etiology of GC and treatment resistance, with a focus on the underlying mechanisms and clinical implications. Research that

Capsaicin is the primary capsaicinoid compound responsible for the spiciness of chilli peppers. Several known and unknown genes synthesize capsaicin through various metabolic pathways, such as the phenylpropanoid or the L-valine metabolism pathways. We conducted comprehensive comparative genomics and proteomics analyses to identify genes and proteins associated with the capsaicin pathway in Capsicum chinense, Capsicum baccatum and the two C.annuum cultivars, CM334 and ECW. A BLAST search against the NCBI database identified 26 and 58 enzyme genes and proteins, respectively. These enzyme genes

Although several studies have investigated the effect of either probiotic feed additives or postmortem meat treatment on the quality of obtained chicken meat, the impact of combined treatment with probiotic feed additives along with meat dipping in essential oil nano-emulsion on meat shelf-life is barely examined. There-fore, this study investigated the effect of combined treatment with Saccharomyces cerevisiae yeast (SCY) and rosemary oil nano-emulsion (RNE) on the quality and shelf-life of chilled broiler meat. The experimental part consisted of adding SCY as a feed additive to broiler

Cotton (Gossypium spp.) is an economically important crop, but its productivity is often hindered by the soil-borne pathogen Verticillium dahliae. This study aimed to investigate the response of cotton roots to V. dahliae infection by analysing the proteome of Gossypium thurberi (resistant) and Gossypium raimondii (susceptible) at 0 h, 24 h, and 48 h post-infection. Through weighted protein coexpression network analysis, fifteen hub proteins crucial for defense against V. dahliae were identified. Expression analysis revealed the pivotal role of GthGAPC2, encoding GLYCERALDEHYDE-3-PHOSPHATE

Chickpea (Cicer arietinum) is a grain crop that is an important source of protein, vitamins, carbohydrates and minerals. It is highly sensitive to salt stress, and salt damage to cellular homeostasis and protein folding affects production. Plants have several mechanisms to prevent cellular damages under abiotic stresses, such as proteins in the endoplasmic reticulum (protein isulfide somerases (PDIs) and PDI-like proteins), which help prevent the build-up of mis-folded proteins that are damaged under abiotic stresses. In this study, we completed initial comprehensive genome-wide analysis of

Jojoba shrubs are wild plants cultivated in arid and semiarid lands and characterized by tolerance to drought, salinity, and high temperatures. Fungi associated with such plants may be attributed to the tolerance of host plants against biotic stress in addition to the promotion of plant growth. Previous studies showed the importance of jojoba as jojoba oil in the agricultural field; however, no prior study discussed the role of jojoba-associated fungi (JAF) in reflecting plant health and the possibility of using JAF in biocontrol. Here, the culture-independent and culture-dependent approaches

Gallbladder cancer (GBC) is an aggressive and lethal malignancy with a poor prognosis. Long noncoding RNAs (lncRNAs) and natural products have emerged as key orchestrators of cancer pathogenesis through widespread dysregulation across GBC transcriptomes. Functional studies have revealed that lncRNAs interact with oncoproteins and tumor suppressors to control proliferation, invasion, metastasis, angiogenesis, stemness, and drug resistance. Curcumin, baicalein, oleanolic acid, shikonin, oxymatrine, arctigenin, liensinine, fangchinoline, and dioscin are a few examples of natural compounds that